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. 2004 May 17;199(10):1421–1431. doi: 10.1084/jem.20040191

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Ex vivo intracellular IFN-γ production by EBNA1-specific T cells after stimulation with LCLs. PBMCs from an HLA B35⁺ donor were incubated alone (A) or with autologous LCL (B), 0.221.B35 LCL (C), HLA B35⁻ LCL (D), or 0.221 LCL (E). Samples shown were gated on the CD8⁺ population, and then the percentage of CD8⁺ and HPV tetramer⁺ cells that were producing IFN-γ was assessed. The percentage of HPV-specific T cells producing IFN-γ after LCL stimulation is shown on the top right hand corner of each of the panels. These data are a representation of two separate experiments.