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. 2004 May 17;199(10):1421–1431. doi: 10.1084/jem.20040191

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — CTL recognition of endogenously processed EBNA1 epitopes. (A) HLA B35⁺ and HLA B35⁻ LCLs were used as targets in a standard ⁵¹Cr-release assay to assess endogenous processing of EBNA1. (A) A CTL clone specific for the HLA B35-binding HPVGEADYFEY epitope was added to target cells at the E/T ratios indicated. (B) An HLA B35⁺ LCL, 721.221 LCLs, 721.221 LCLs transfected with HLA B*3501, T2 LCLs, and T2 LCLs transfected with HLA B*3501 were used as targets in a standard ⁵¹Cr-release assay to assess CTL activity to an HPVGEADYFEY-specific CTL clone at an E/T ratio of 5:1. These data are a representation of three separate experiments.