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. 2004 May 17;199(10):1305–1315. doi: 10.1084/jem.20031132

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — KLF2 interacts directly with p300. (A) p300 rescues KLF2-mediated inhibition. Transient transfection studies with the indicated plasmids were performed in COS-7 cells. Cotransfection studies demonstrate that p300 can rescue KLF2 and KLFΔZnF-mediated inhibition of the NF-κB concatemer. n = 6–12 per group. *P < 0.00001; **P < 0.00002. (B) KLF2 and p300 cooperate to induce the eNOS promoter. Cotransfection studies were performed in COS-7 cells. Cotransfection of KLF2 and p300 induces eNOS promoter activity greater than either factor alone. n = 6–12 per group; *P < 0.001; **P < 0.0001; ^#P < 0.05. (C) KLF2 and p300 interact. GST fusion proteins were generated for KLF2 and p300. Both KLF2 and KLFΔZnF can interact with p300 (top). Conversely, KLF2 interacts specifically with the NH₂ terminus of p300. (D) KLF2 and p300 interact in cells. COS-7 cells were transfected with HA-KLF2 and Flag-p300. Immunoprecipitation was performed using the α-Flag antibody (or isotype control) followed by Western blot for KLF2 with α-HA antibody.

Figure 5. — KLF2 interacts directly with p300. (A) p300 rescues KLF2-mediated inhibition. Transient transfection studies with the indicated plasmids were performed in COS-7 cells. Cotransfection studies demonstrate that p300 can rescue KLF2 and KLFΔZnF-mediated inhibition of the NF-κB concatemer. n = 6–12 per group. *P < 0.00001; **P < 0.00002. (B) KLF2 and p300 cooperate to induce the eNOS promoter. Cotransfection studies were performed in COS-7 cells. Cotransfection of KLF2 and p300 induces eNOS promoter activity greater than either factor alone. n = 6–12 per group; *P < 0.001; **P < 0.0001; ^#P < 0.05. (C) KLF2 and p300 interact. GST fusion proteins were generated for KLF2 and p300. Both KLF2 and KLFΔZnF can interact with p300 (top). Conversely, KLF2 interacts specifically with the NH₂ terminus of p300. (D) KLF2 and p300 interact in cells. COS-7 cells were transfected with HA-KLF2 and Flag-p300. Immunoprecipitation was performed using the α-Flag antibody (or isotype control) followed by Western blot for KLF2 with α-HA antibody.