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. 2004 May 17;199(10):1445–1450. doi: 10.1084/jem.20030635

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Complementation of sialylation deficiency of TbGPI8-KO mutant by soluble form trans-sialidase. (A) Partial restoration of sialic acids on the surface of GPI8KO by expressing soluble form trans-sialidase. The amount of sialic acids on wild-type (WT), GPI8KO transfected with an empty vector (mock), and GPI8KO-expressing soluble form trans-sialidase (sTS) were determined. Standard deviation of three independent measurements is indicated. (B) Restoration of the surface sialic acids rescued infectivity to Tsetse flies. Infectivity was scored in a similar way as described in Fig. 1 A. The number of flies in each group is indicated above each bar. (C) Different infectivities of sTS-transfected trypanosomes in male and female flies.