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. 2004 Feb 16;199(4):459–470. doi: 10.1084/jem.20031219

Figure 2.

Figure 2.

Characterization of EBNA1-specific T cells. (A) Recognition of LCL 111 by T cell clones derived from the M3-W1 T cell line. (B) FACS® analysis of M3-W1-B9 T cells for CD8 expression. T cells were stained with anti–CD4-PE or anti–CD8-FITC. Positive staining for CD8 T cells is shown as an open histogram and control antibody staining is represented as a shaded histogram. (C) Identification of minimal EBNA1 T cell epitope for MHC class I binding. Four different peptides were made and pulsed onto 1359mel cells at 10 μM concentration. After washing, the peptide-pulsed cells were cocultured with T cells overnight. IFN-γ release was determined from culture supernatants. (D) EBNA1-P518–526 peptide titration experiment for M3W1-B9 T cell recognition. EBNA1-P518–526 peptide at various concentrations were pulsed on 1359mel cells and used as target cells to stimulate T cells. A control peptide EBNA1-P 572–584 was also used at various concentrations. Experiments were repeated twice with similar results.