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. 2004 Feb 16;199(4):459–470. doi: 10.1084/jem.20031219

Figure 4.

Figure 4.

HLA-B8 molecule functions as a restriction element for M3-W1-B9 CD8+ T cells. (A) T cell recognition of peptide-pulsed HLA-B8–expressing cell lines. (B) Identification of HLA-B8 molecule as a restriction element for T cell recognition. HEK293 cells cotransfected with HLA-B8 plus full-length EBNA1-GFP or EBNA1-GAr-del-GFP cDNAs (with GAr domain deleted) were tested for recognition by M3-W1-B9 CD8+ T cells. Positive and negative signs indicate cotransfection of target cells in the presence or absence of HLA-B8 cDNA, respectively. (C) Natural processing and presentation of the native form of EBNA1 for T cell recognition. HEK293 cells cotransfected with full-length EBNA1 and HLA-B8 cDNAs were cocultured with M3-W1-B9 CD8+ T cells overnight. IFN-γ secretion from T cells was determined by ELISA. (D) Endogenous generation of HLA-B8–restricted EBNA1 peptide for T cell recognition. HEK293 cells transfected with HLA-B8 cDNA were mixed with HEK293 cells transfected with EBNA1-GFP cDNA at a 1:1 ratio. The mixed cells were then cocultured with M3-W1-B9 CD8+ T cells overnight. IFN-γ release from CD8+ T cells was measured from culture supernatants.