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. 2004 Feb 16;199(4):459–470. doi: 10.1084/jem.20031219

Figure 5.

Figure 5.

Specific lysis of HLA-B8–matched EBV-transformed LCLs by M3-W1-B9 CD8+ T cells. (A) Recognition of HLA-B8–matched LCLs by M3-W1-B9 CD8+ T cells. LCLs were cocultured with M3-W1-B9 CD8+ T cells at an E/T ratio of 1:1. Mismatched LCLs were used as negative controls. (B) Specific lysis of HLA-B8–matched LCL 111 cells by CD8+ T cells at different E/T ratios. LCL 1 was used as a negative control. LCL cells were labeled with 51chromium. Cytolysis by CD8+ T cells was determined in a 16-h chromium release assay. (C) Cold target inhibition of recognition of LCL 111 cells by M3-W1-B9 CD8+ T cells. Lysis of LCLs by M3-W1-B9 CD8+ T cells was specifically inhibited when EBNA1-P518–526–pulsed cold LCL 111 targets were used. Lysis was tested with an effector to hot target ratio of 40:1. Cold LCL 111 target cells were pulsed with EBNA1-P518–526 or EBNA1-P572–584 peptide at a concentration of 1 μM and were mixed with hot targets at a ratio of 4:1. All experiments were repeated twice with similar results.