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. 2004 Feb 16;199(4):459–470. doi: 10.1084/jem.20031219

Figure 7.

Figure 7.

Generation of EBNA1-P518–526 peptide–specific T cells from HLA-B8–expressing PBMCs. (A) Detection of EBNA1-P518–526 peptide–reactive T cells from HLA-B8+ donor PBMCs. 105 PBMCs were seeded per well and experiments were performed in quadruplicate wells. An HLA-A2–restricted NY-ESO-1 peptide served as a control. HLA-mismatched donor 5 and an HLA-B8+ PMBC donor 4 that is seronegative for EBV were also included. (B) Recognition of LCL 1088 by CD8+ T cell clones from the PBMCs of donor 3. T cells generated from PBMCs were stimulated with EBNA1-P518–526 peptide as described in Fig. 1. Six CD8+ T cell clones were generated from two T cell lines and were capable of recognizing HLA-B8–expressing LCL 1088 target cells. (C) D1-B11 CD8+ T cell recognition of HLA-B8–matched LCLs. LCLs were cocultured with T cells at an E/T ratio of 1:1. All experiments were repeated twice with similar results.