Figure 10.
Lack of presentation of soluble ovalbumin by bone marrow–derived PDCs. BM cultures were pulsed on day 10 with 500 μg /ml of soluble ovalbumin in the presence or absence of CpG. MDC and PDCs were sorted on day 11. C57BL/6 mice (n = 3) were primed by intravenous injection of 105 cells and boosted after 10 d with vaccinia virus encoding full-length ovalbumin. (A) CTL responses were assessed in the blood by FACS® analysis using SIINFEKL-H-2-Kb tetramers 7 d after boosting. Mean proportions of tetramer+ cells as a percentage of CD8 cells (± SEM) for each group are shown. (B) IFN-γ ELISPOT was performed on splenocytes to assess responsiveness to ovalbumin MHC class I (SIINFEKL) and class II–restricted peptides (each at 10 μg/ml) 10 d after boosting. All animals showed comparable responses to PHA stimulation (not depicted).