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. 2004 Apr 5;199(7):947–957. doi: 10.1084/jem.20031389

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Increased frequency of cytokine-secreting, sulfatide-reactive lymphocytes after immunization with sulfatide. Groups of mice (two in each) were immunized in complete Freund's adjuvant with 20 μg of sulfatide or with 20 μg of mono-GM1 or with PBS/vehicle alone. 10 d later, splenocytes were isolated, recalled in vitro with sulfatide, and subjected to ELISPOT analysis as described in Materials and Methods. The average number of spots/10⁶ spleen cells for both IFN-γ and IL-4 in each group is shown.