Figure 7.

Inhibition of cytokine secretion by MOG-reactive T cells after coinjection with sulfatide. Groups of female BL/6 mice were coinjected i.p. with either 20 μg of sulfatide in PBS/vehicle or PBS/vehicle alone at the time of s.c. challenge with 100 μg MOG_35–55 in CFA. 10 d later, LN cells were cultured with graded concentrations of MOG_35–55 or protein-purified derivative of Mycobacterium (PPD) for the measurement of proliferation and cytokine secretion by ELISA and ELISPOT analysis. Averaged responses within the group are plotted. A representative of three independent experiments is shown. *P-value < 0.004.