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. 2004 Apr 19;199(8):1089–1099. doi: 10.1084/jem.20040051

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — CD4 gene silencing in TOX-Tg mice. (A) RT-PCR analysis for expression of the indicated genes in purified CD8SP or DP thymocytes isolated from TOX-Tg/MHC^o mice. (B and C) Western blot analysis for expression of Runx proteins in whole cell lysates prepared from total thymocytes or isolated CD8SP cells as indicated. Expression of β-actin was used as a loading control.