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. 2004 Apr 19;199(8):1041–1052. doi: 10.1084/jem.20031080

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — DISC analysis in HRS cell lines. (A) Whole cell lysates of different nonHodgkin and Hodgkin cell lines were analyzed for expression of CD95, FADD, C-8 (p55/p53C-8), and C-10 (p59/p55C-10) by Western blot. As control, α-tubulin expression is shown. (B) DISC analysis in H9 cells and HRS cell lines by CD95 immunoprecipitation. The indicated cell lines were incubated for 10 min with the agonistic anti-CD95 mAb APO-1. Thereafter, cells were lysed and immunoprecipitation was performed. The precipitates were washed, subjected to 10% SDS-PAGE, and analyzed by Western blot for coimmunoprecipitation of FADD, C-8, C-10, and c-FLIP, as indicated.