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. 2004 May 3;199(9):1213–1221. doi: 10.1084/jem.20031462

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — Chemokine expression in primary untreated neuroblastomas. (A) Expression profiles were generated from 79 untreated stage 4 neuroblastomas from patients using Affymetrix U95Av2 oligonucleotide microarrays. All genes (black) are plotted according to their average level of expression in tumors with undetectable (x axis) versus detectable (y axis) Vα24-Jα18 RNA (iNKT TCR). Gene probe sets for 43 chemokines are enlarged and labeled in red. Yellow dashed lines mark 95% confident interval. Names of chemokine genes that positively correlate with iNKT RNA expression are shown. (B) A tumor that expressed CCL2 mRNA was immunostained with goat anti–human CCL2 followed by the Cell and Tissue Staining Kit (R&D Systems). Tumor cells expressing CCL2 have brown cytoplasm.