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. 2004 May 3;199(9):1201–1211. doi: 10.1084/jem.20031706

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Copyright © 2004, The Rockefeller University Press

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Figure 7. — Sec22b function is important for biogenesis of the Legionella replicative vacuole. (A and B) CHO cells were cotransfected with plasmids encoding FcγRII and the proteins indicated on the x axis of the graph. (A) Growth of IgG-opsonized Legionella was measured over a 24-h period by measuring the fold increase in CFUs. Values were normalized to control cells that received a plasmid encoding GFP. Values are the mean ± SE for three independent assay wells. (B) SEAP assays demonstrate that overproduction of either Membrin or VAMP4 interferes with alkaline phosphatase secretion by a process that is not suppressed by Sec22b overproduction. Values are the mean ± SE for three independent assay wells. (C) CHO FcγRII cells were cotransfected with plasmids encoding Sec22b and Membrin. The ratio of Sec22b:Membrin plasmid DNA used in each transfection is indicated on the x axis. Data show that delivery of Sec22b to the LCV is inhibited as the amount of plasmid encoding Membrin increases. Data represent the mean ± SE for three independent assay wells in which at least 20 vacuoles were scored in each assay.