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. 2004 May 3;199(9):1235–1244. doi: 10.1084/jem.20040373

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — SHM by NES mutant AID in E. coli. (A) AID, AID^F198A, and AID^L62A (a catalytically inactive control; reference 25) protein expression in E. coli measured by Western blotting with rabbit anti–AID antiserum. (B) SHM measured by Kan-resistance assay for AID-mediated cytidine deamination in UDG (uracil DNA glycosylase)-deficient E. coli. Circles represent the mutation frequency of individual starting colonies and horizontal bars represent mean values. Results from two independent experiments are shown. Mean frequencies of Kan^R colonies are indicated underneath each group.