Figure 2.

T cell–specific deletion of the IL-10 gene in IL-10 ^FL/FL CD4-Cre⁺ mice. Southern blot analysis of DNA extracted from different FACS-sorted cell populations and tissues after PstI digest. (A) CD3⁺ T and CD19⁺ B cells sorted to a purity of 98 and 96%, respectively, from splenocyte suspension of a 3-mo-old mouse. The result is representative of five animals. (B) F4/80⁺ macrophages from peritoneal lavage fluid (purity 97%, representative of four animals) and splenic T cells. (C) Splenic CD11c⁺ dendritic cells sorted to a purity of 95% (representative of three animals) and splenic T cells. (D) Various tissues and splenic T cells from an 8-wk-old mouse.