Figure 1.

Analysis of monocyte trafficking and microsphere transport to LNs in CX₃CR1^gfp/gfp mice. (A) Monocyte subsets were identified in blood of CX₃CR1^gfp/gfp mice as GFP⁺ cells with differing levels of Gr-1, revealing the presence of a small Gr-1^int subpopulation in addition to previously described Gr-1^hi and Gr-1^lo populations. (B) GFP fluorescent intensities of these subsets, stable and noninterconverting for at least 1 d (4), were compared with the fluorescent intensities of cells that engulfed red fluorescent microspheres deposited in skin, examined 14 h after injection, and in cells that emigrated from skin to LNs by day 2. The same settings in flow cytometry were used to acquire all of these data, making them directly comparable. (C) Profiles of the cells in skin at the site of microsphere injection at 14 h are shown. (D) Quantification and profiles of LX-bearing cells that migrated to LNs were analyzed at day 2. To combine data from different experiments, the mean number of migrated cells in WT mice was set equal to 1.0 for each experiment, and relative values for all WT and knock-out individuals in that experiment were calculated. Six mice were studied for each part of the figure over the course of two experiments with three mice in each group.