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. 2004 Nov 15;200(10):1231–1241. doi: 10.1084/jem.20032152

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Analysis of monocyte subsets in blood: expression of costimulatory activity and CCR mRNA. (A) Sorted Gr-1^hi, Gr-1^int, and Gr-1^lo subsets were irradiated and used as APCs in an MLR with BALB/c T cells. This experiment is representative of two conducted experiments, both showing similar results. (B) Real-time PCR for CCR2, CX₃CR1, CCR7, and CCR8 mRNA was conducted. Data are plotted as the percentage of expression relative to the subset with the highest expression of a particular CCR. These data were repeated in a similar pattern in three independent sorting experiments.