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. 2004 Nov 15;200(10):1267–1278. doi: 10.1084/jem.20040500

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Complementation of B. henselae BadA⁻. (a) Detection of BadA expression in B. henselae WT, Pil⁻, BadA⁻, and BadA⁻/BadA⁺ WT by TEM. Note the brush-like arrangement of BadA on the surface of the WT and the complemented mutant (arrows) missing in the Pil⁻ variant and the BadA⁻ mutant. (b) Detection of BadA expression by immunofluorescence using an anti-BadA rabbit serum. Bar, 2 μm. (c) Detection of BadA expression by IEM using 10 nm gold-conjugated goat anti–rabbit IgG.