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BadA-dependent interaction of B. henselae with ECM proteins. Collagen type I, III, and IV, and laminin and Fn binding of B. henselae WT, Pil−, BadA−, and BadA−/BadA+ WT. (a) Bacteria were seeded onto coated coverslips and DAPI stained. Bar, 20 μm. Fn binding was additionally assessed by Western blotting. (b) Microscopic quantification of bacterial binding to ECM proteins (adherent bacteria per high power field). *, significant difference compared with B. henselae WT (P < 0.01).
