Table II.
Plasmids, Primers, and Probes Used in This Study
| Plasmids | Description | Reference or source |
|---|---|---|
| pBluescript II KS | cloning vector, Apr | Stratagene |
| pET30b | protein expression vector, Kmr | Novagen |
| pBBR1MCS | broad host range, Cmr | 46 |
| pTR14 | pBluescript II KS containing a 13.2-kb B. henselae Marseille EcoRI/ClaI fragment with badA, Apr |
This study |
| pTR15 | pBBR1MCS containing a 13.2-kb BamHI/ClaI fragment of pTR14 with badA, Cmr |
This study |
| pTR52 | pET30b containing badA-f6/r6-PCR fragment | This study |
| Primers | ||
| badA-f2 | CTCCAGTCTGATGATTCAGC | This study |
| badA-r2 | GCTATATTGATTTCAGTACCTGC | This study |
| badA-f6 | TGCACATATGAAAGCATTAAGGGGAATGATATCAGa | This study |
| badA-r6 | TTATCTCGAG TCAAGTACGCTTATCACTTTTGTTATTAGCb | This study |
| Probe | ||
| pilin | GNTTYTTNAARGAYGARTCNGGNGCANCNGCNATHGARTAYG GNCTNATHGCNGCNTTNATHTCNGT |
This study |
a
Italic, NdeI restriction site.
b
Italic, XhoI restriction site; underline, stop codon.