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. 2004 Aug 16;200(4):411–423. doi: 10.1084/jem.20040845

Table IV.

Statistical Analysis of Data Obtained from Adoptive Transfers of NG × H2-SVEX Transgenic Bone Marrow: Young→Aged and Young→Young

Significance of differences Young recipienta Aged recipienta p-value
% of donor pro–B cells that are GFP+ 71 ± 5.1 37 ± 21 <0.001
% of donor pro–B cells that are VEX+ 33 ± 4.8 17 ± 6.4 <0.001
Pre:pro ratiob 3.2 ± 0.56 0.66 ± 0.56
Log of pre:pro ratioc 1.1 ± 0.18 −0.83 ± 1.0 <0.001
Correlations b/t characteristics,
all mice (n = 30)		 log pre:pro ratio % of pro–B cells
that are GFP+ 		% of donor pro–B cells
that are VEX+
Log pre:pro ratio r = 0.812, P < 0.001 r = 0.860, P < 0.001
% of pro–B cells that are GFP+ r = 0.812, P < 0.001 r = 0.835, P < 0.001
% of pro–B cells that are VEX+ r = 0.860, P < 0.001 r = 0.835, P < 0.001
Correlations b/t characteristics,
aged recipient mice (n = 17)		 log pre:pro ratio % of pro–B cells
that are GFP+ 		% of donor pro–B cells
that are VEX+
Log pre:pro ratio r = 0.550, P = 0.022 r = 0.670, P = 0.003
% of pro–B cells that are GFP+ r = 0.550, P = 0.022 r = 0.634, P = 0.006
% of pro–B cells that are VEX+ r = 0.670, P = 0.003 r = 0.634, P = 0.006

CD45.1 NG × H2-SVEX double transgenic mice (GFP serves as a reporter of rag2 expression, and VEX serves as a reporter of V(D)J recombinase activity) were used as donors for adoptive transfer. Bone marrow was harvested from wild-type young (3–4 mo at time of harvest) and aged (23–29 mo at time of harvest) CD45.2 recipient mice 5–6 wk after adoptive transfer of bone marrow from young (1–3 mo) NG × H2-SVEX CD45.1 mice. Pro–B (IgM−B220+CD43+) and pre–B cells (IgM−B220+CD43−) of donor origin were identified based on expression of CD45.1, using flow cytometry as described in Materials and Methods.

a

Means ± SD are presented.

b

The pre:pro ratio was calculated by dividing the number of pre–B cells by the number of pro–B cells. Numbers of pro–B and pre–B cells in bone marrow were determined by multiplying the frequency by the leg bone marrow cell number determined by counting to obtain total numbers.

c

Log of the pre:pro–B ratio was used because its distribution was normal, whereas the ratio was not.

n, number of mice; r, correlation coefficient.