Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Dec 6;200(11):1383–1393. doi: 10.1084/jem.20040795

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Absence of SIGN-R1 does not impair natural antibody levels or opsonization of S. pneumoniae. (a) Natural anti-PC antibody levels in control and SIGN-R1^−/− mice. Sera from naive WT and SIGN-R1^−/− mice were assayed for natural antibodies against PC by ELISA. Each point represents the value from an individual mouse. p-values were determined using an unpaired Student's t test (Welch corrected). (b and c) Nonimmune serum from control and SIGN-R1^−/− mice provide equivalent opsonization. The RAW-297 macrophage cell line (b) or BALB/c peritoneal macrophages (c) were incubated at 37°C with FITC-labeled S. pneumoniae opsonized with heat-inactivated nonimmune serum (NIS) from wild-type or SIGN-R1^−/− mice, followed by flow cytometric analysis. Phagocytosis is expressed as percentage of FITC positive cells. Values represent mean of triplicates, the experiments shown are representative of two, and p-values were obtained using an unpaired Student's t test.