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Apoptotic cells stimulate endothelial cells to secrete IL-8. HCAECs were incubated for 4 h at 37°C with culture media alone; viable, normal thymocytes; or apoptotic thymocytes induced by UV irradiation as stimulant. Apoptotic thymocytes themselves did not release IL-8. 200 ng/ml LPS was used as a positive control. After the incubation, IL-8 in the supernatants was determined as described in Materials and Methods. Data represent the mean ± SEM of quadruplicates.
