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. 2004 Sep 20;200(6):749–759. doi: 10.1084/jem.20040874

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Ki67 expression by memory T cells in the GI tract, LNs, and peripheral blood. Lymphocytes from HIV⁻ (A, B, E, and F) and HIV⁺ (C–F) individuals obtained from the peripheral blood, LNs, and GI tract were stained extracellularly with anti-CD3, anti-CD4 antibodies, and anti-CD8 antibodies followed by intracellular staining with anti-Ki67. The calculated percentage of memory T cells that express Ki67 represents lymphocyte gated, CD3⁺, and either CD4⁺ or CD8⁺ gated events that stain positively with anti–Ki67 and were then expressed as the percentage of memory CD4⁺ or CD8⁺ T cells as described in Materials and Methods.