Figure 9.

CD4⁺CD25⁺ T cells suppress priming of concomitant immunity. RAG1 ^−/− mice were adoptively transferred with various populations of naive T cells from C57BL/6J donor mice as follows: (A) no cells, (B) CD8⁺ and CD4⁺ T cells, (C) CD8⁺ and CD4⁺CD25⁻ T cells, (D) CD8⁺ and CD4⁺CD25⁺ T cells, or (E) CD8⁺ T cells alone. Adoptively transferred mice were inoculated with primary B16 tumors on day 1, followed by challenge tumors on day 7. In each panel, the left graph depicts growth of primary tumors and the right graph depicts growth of challenge tumors in the same mice. In D, the experiment was terminated 8 d after tumor challenge because growth of primary tumors was too rapid. Five out of nine tumors had already appeared by day 9.