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. 2004 Sep 20;200(6):811–816. doi: 10.1084/jem.20040776

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Copyright © 2004, The Rockefeller University Press

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Figure 2. — T224W and G428E perforin localize differently in RBL cells. (A) Immunohistochemistry of perforin-expressing RBL cells demonstrated with antiperforin antibody PI-8 and counterstained with eosin. (B) RBL cells either unlabeled or labeled with α-TNP–IgE were stained as in A, after degranulation was induced by transient incubation with TNP-labeled target cells. Magnification, 400×.