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. 2004 Sep 20;200(6):783–795. doi: 10.1084/jem.20040254

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — BLS cells support the migration and adhesion of T cells and DCs. (a) BLS4 cells express various chemokines with or without TNFα stimulus. Transcripts for the indicated chemokines were detected by semi-quantitative RT-PCR analysis. (b) BLS4 supernatant (sup.) induces the chemotaxis of CD4⁺ T cells. The chemotactic activity in diluted culture supernatants from BLS4 cells (prestimulated or not prestimulated with TNFα) was analyzed by a chemotaxis assay. (c) Chemotaxis of CD4⁺ T cells to the BLS supernatants is Gαi dependent. T cells were pretreated or not pretreated with PTx and subjected to the chemotaxis assay. Two-fold diluted supernatants were used as attractant. (d) BLS4 supernatant induces DC chemotaxis. Immature (iDC) or mature (mDC) DCs were pretreated or not pretreated with PTx and subjected to the chemotaxis assay. (e) BLS4 cells support DC adhesion to their surface. DCs were pretreated or not pretreated with PTx and subjected to the adhesion assay.