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. 2004 Aug 2;200(3):353–365. doi: 10.1084/jem.20040213

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — Recruitment of Fas and downstream signaling molecules into membrane rafts after ET-18-OCH₃ treatment. Untreated Jurkat cells (Control) and Jurkat cells treated with 10 μM ET-18-OCH₃ (ET-18-OCH₃) for 6 h were lysed in 1% Triton X-100 and subjected to discontinuous sucrose density gradient centrifugation. Individual fractions were subjected to SDS-PAGE and Western blotting. Location of G_M1-containing rafts (fractions 3–6) was determined using CTx B subunit conjugated to horseradish peroxidase. Location of the indicated receptors and signaling proteins were examined using specific antibodies. Active p18 caspase-8 and p23 caspase-10 cleavage forms are indicated. Representative blots of three separate experiments are shown.