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. 2004 Aug 2;200(3):353–365. doi: 10.1084/jem.20040213

Figure 5.

Figure 5.

Figure 5.

Clustering of Fas-deficient rafts is not sufficient for apoptosis. (A) Cell surface expression of Fas, TNFR1, and DR5 in Jurkat (JK) and Fas-deficient Jurkat cells was determined by flow cytometry. Percent of positive cells for each death receptor was estimated using the P3X63 (X63) myeloma culture supernatant as negative control. (B) Induction of apoptosis in Jurkat (JK) and Fas-deficient Jurkat cells was determined by flow cytometry after a 24-h incubation with 50 ng/ml cytotoxic anti-Fas CH-11 mAb, 100 ng/ml rhFasL (FasL), or 10 μM ET-18-OCH3 (ET). Untreated control cells (C) were run in parallel. Data shown are means ± SD of three independent experiments. (C) Fas-deficient Jurkat cells were untreated (Control) or incubated with 10 μM ET-18-OCH3 (ET-18-OCH3) for 6 h, and then stained with FITC-CTx B subunit and analyzed by confocal microscopy to visualize membrane rafts. Images shown are representative of three independent experiments. Bar, 10 μm.