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. 2004 Aug 2;200(3):353–365. doi: 10.1084/jem.20040213

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Copyright © 2004, The Rockefeller University Press

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Figure 7. — Selective incorporation of the fluorescent analogue PTE-ET-18-OCH₃ in human leukemic cells. Human leukemic Jurkat cells were incubated with the indicated concentrations of ET-18-OCH₃ or PTE-ET-18-OCH₃ for 24 h and apoptosis was measured by flow cytometry (A). Normal PBLs and leukemic HL-60 and Jurkat cells (JK) were treated with 20 μM PTE-ET-18-OCH₃ for 7 h, washed with 1% BSA-PBS, and fluorescent drug uptake was analyzed by fluorescence microscopy (blue fluorescence) (B) or by fluorescence quantitation (C). Data shown are means ± SD of three independent determinations.

Figure 7. — Selective incorporation of the fluorescent analogue PTE-ET-18-OCH₃ in human leukemic cells. Human leukemic Jurkat cells were incubated with the indicated concentrations of ET-18-OCH₃ or PTE-ET-18-OCH₃ for 24 h and apoptosis was measured by flow cytometry (A). Normal PBLs and leukemic HL-60 and Jurkat cells (JK) were treated with 20 μM PTE-ET-18-OCH₃ for 7 h, washed with 1% BSA-PBS, and fluorescent drug uptake was analyzed by fluorescence microscopy (blue fluorescence) (B) or by fluorescence quantitation (C). Data shown are means ± SD of three independent determinations.