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. 2004 Aug 2;200(3):297–306. doi: 10.1084/jem.20031435

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — T2 binding assay identifies mesothelin protein-derived epitopes that bind to HLA-A2, A3, and A24 molecules. T2 cells were pulsed with 0–225 μg/ml of peptide overnight at room temperature before analysis by flow cytometry. (A) T2 cells expressing HLA-A2 and pulsed with either a mesothelin A1_(309–317) peptide (closed diamond), mesothelin A2_(20–28) (closed square), and mesothelin A2_(530–538) (closed triangle). (B) T2 cells genetically modified to express HLA-A3 and pulsed with either mesothelin A1_(309–317) peptide (closed diamond), mesothelin A3_(83–91) (closed square), and mesothelin A3_(225–233) (closed triangle). (C) T2 cells genetically modified to express HLA-A24 and pulsed with either mesothelin A1_(309–317) peptide (closed diamond), mesothelin A24_(435–443) (closed square), and mesothelin A24_(475–483) (closed triangle).