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. 2004 Aug 2;200(3):297–306. doi: 10.1084/jem.20031435

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Expression of surface mesothelin of both the Panc 6.03 and Panc 10.05 vaccine and on cell lines used as targets for CTL assays. Panc 6.03, Panc 10.05, autologous EBV, autologous EBV transduced with mesothelin, Panc 2.5 (HLA-A3⁺), Panc 3.014 (HLA-A3⁺), and Panc 3.11 (HAL-A3⁻) were analyzed by flow cytometry for their levels of surface mesothelin using the mesothelin-specific monoclonal antibody CAK1 as the primary antibody and goat anti–mouse IgG1 FITC as the secondary antibody. The dotted line represents the isotype control, and the solid line represents mesothelin staining.