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. 2004 Aug 2;200(3):277–285. doi: 10.1084/jem.20040165

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Effect of depletion of CD4⁺CD25⁺ T cells from PBMCs on TNFα and IL-10 production from the same patient before and after treatment. Total PBMCs or PBMCs depleted of CD25⁺ cells (by MACS^® sorting) were stimulated with 5 μg/ml of soluble anti-CD3/anti-CD28 for 48 h. 2 μM monensin was added for the last 5 h of culture. Staining of CD4 and CD25 surface expression was performed. Cells were permeabilized and stained with PE-conjugated anti-TNFα or anti–IL-10. Results from three patients before and after therapy and three healthy individuals are shown.