Figure 6.

Only CD4⁺CD25⁺ regulatory T cells derived from patients treated with anti-TNFα, but not from patients with active RA, convey a suppressive phenotype to effector T cells. MACS^®-sorted CD4⁺CD25⁺ T cells and CD4⁺CD25⁻ T cells were cultured either alone or mixed at a 1:1 ratio (10⁵ cells/well) and stimulated with 1 μg/ml of soluble anti-CD3 and 2 μg/ml anti-CD28. After 5 d of culture, CD4⁺CD25⁺ T cells were depleted and the remaining CD4⁺CD25⁻ T cells were replated 1:1 with freshly isolated autologous CD4⁺CD25⁻ T cells in the presence of anti-CD3 and anti-CD28 for an additional 3 d (third column). ³[H]Tdr was added for the final 8 hof culture (expressed as mean ± SEM of triplicate cultures). Results from three patients before and after therapy and three healthy individuals are shown.