Figure 3.

Reovirus T1L in association with double negative DCs (CD11c⁺/CD8α⁻/CD11b^lo) in vivo. Cytospin preparations of CD11c⁺ DCs isolated from PPs of mice 24 h after peroral inoculation with T1L. Cells were stained with antibodies specific for reovirus σ1 protein and cell surface molecules, and examined by confocal microscopy. Nuclei were labeled with Hoechst (blue). (A) Anti-CD8α (green) and anti-σ1 (red). Reovirus is found in association with CD8α⁻ cells. (B) Anti-CD11b (green) and anti-σ1 (red). Reovirus antigen is found in association with CD11b^lo cells. (C) Anti-B220 (red) and anti-σ1 (green). Reovirus antigen is found in B220⁻ cells, indicating that it is not within plasmacytoid DCs.