Figure 6.

Reovirus T1L antigen in association with activated caspase-3 and epithelial cell–derived cytokeratin in CD11c⁺ DCs isolated from reovirus-infected PPs and purified by flow cytometry. Cytospin preparations were obtained from mice 24 h after peroral infection with T1L, stained, and examined by confocal microscopy. Nuclei were labeled with Hoechst (blue). Staining for (A) reovirus σ1 (green) and (B) activated caspase-3 (red) indicates that the majority of virus⁺ cells also express active caspase-3 within vesicular structures as indicated by the yellow color in the merged image (C). Expression of active caspase-3 in T1L-infected cells also is shown in D: reovirus σ1 (red), activated caspase-3 (green), and CD11c (gray). (E) Staining of cells for cytokeratin (green), reovirus σ1 (red), and CD11c (gray) demonstrates that reovirus σ1 also colocalizes with cytokeratin within vesicular structures as indicated by the yellow color. In this micrograph, a CD11c⁺ cell containing cytokeratin without reovirus is evident (large arrow), as is a cytokeratin-expressing contaminating epithelial cell (small arrow). Note that the cytokeratin in this cell stains diffusely and is not punctate.