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. 2005 Jun 20;201(12):2011–2021. doi: 10.1084/jem.20050042

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — UNG2-F251S is abnormally translocated to mitochondria. (A) WT UNG2-EYFP or UNG2-F251S-EYFP transfected in HeLa cells and followed in live cells for 2 d. (B) Control coexpression of WT UNG2 with different fluorescent tags (upper row). UNG2-F251S and WT UNG2 are localized different in human cells, demonstrated by coexpression of UNG2-F251S-EYFP and WT UNG2-ECFP in the same cell (lower row). (C) Coexpression of WT UNG1-ECFP and UNG2-EYFP demonstrates distinct sorting to mitochondria and nuclei, respectively (upper row), whereas coexpression of mutant UNG2-F251S-EYFP and UNG1-ECFP abolishes translocation of the mutant to nuclei, while increasing its accumulation at mitochondria. (D) UNG2-F251S-EYFP but not WT UNG2-EYFP colocalizes with mitochondria in fixed cells as demonstrated using an antibody against human mitochondria.