Table 1.

Venular Microhemodynamics and Rolling Velocities of LNCs

mAb inhibition		No. Venules/ No. animals		Diameter		Vfast		Vblood		WSR (WSS)		Vroll (Median)		Vrel (Median)
				μm		μm/s		μm/s		s−1 (dyne/cm2)		μm/s		%
Control		3/3		38.4 ± 5.9		1236 ± 603		1105 ± 456		228 ± 75 (5.6 ± 1.9)		38.6 ± 21.7 (14.7)		3.4 ± 2.8 (2.0)
L-selectin		3/3		38.4 ± 5.9		1099 ± 266		1168 ± 486		250 ± 59 (6.3 ± 1.5)		N/A		N/A
Control		4/4		36.2 ± 8.5		999 ± 521		1150 ± 309		267 ± 82 (6.7 ± 2.1)		24.2 ± 18.6 (10.0)		2.7 ± 1.8 (0.9)
α4 integrin		4/4		26.2 ± 8.5		1456 ± 673		1297 ± 475		289 ± 70 (7.2 ± 1.7)		34.1 ± 29.5 (15.8)		2.7 ± 2.7 (1.3)
Control		3/3		32.7 ± 4.8		1103 ± 482		1021 ± 386		248 ± 71 (6.2 ± 1.8)		43.0 ± 34.9 (32.1)		4.5 ± 4.0 (3.5)
LFA-1		3/3		32.7 ± 4.8		1109 ± 462		1149 ± 492		273 ± 81 (6.8 ± 2.0)		44.9 ± 37.6 (30.8)		4.4 ± 3.8 (2.6)
MTX		3/3		36.9 ± 5.0		1592 ± 815		1392 ± 949		319 ± 91 (8.0 ± 2.3)		64.1 ± 40.4 (23.2)		4.9 ± 3.7 (1.4)
PTX		3/3		36.9 ± 5.0		1792 ± 809		1569 ± 662		332 ± 99 (8.3 ± 2.5)		66.4 ± 48.6 (33.9)		5.2 ± 5.0 (1.9)

Mean velocities of noninteracting (V_fast) and rolling (V_roll) LNCs in one representative order III venule per animal were measured. These data together with the venular diameter were used to calculate V_blood and wall shear rate (WSR) as described in Materials and Methods. The wall shear stress (WSS) was estimated based on the assumption that the blood viscosity in HEVs is 0.025 Poise. To correct for hemodynamic differences between control and teatment periods in individual venules that may have mechanically affected LNC rolling behavior, V_roll was also expressed as percentage of V_fast (V_rel). Anti–L-selectin treatment essentially eliminated LNC rolling in HEVs. Thus, no data for V_roll and V_rel were obtainable. Data shown are mean ± SD. Since velocity histograms of V_roll indicate that data were not normally distributed (see Fig. 5), median values are shown as well.