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. 1998 Jan 19;187(2):147–159. doi: 10.1084/jem.187.2.147

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Adoptively transferred p52-deficient lymphocytes support formation of germinal centers in RAG–1-deficient mice. Lethally irradiated RAG-1–deficient mice were injected with bone marrow cells isolated from 11-wk-old p52 (−/−), (+/−), and (+/+) donor mice, and 15 wk later challenged intraperitoneally with TNP-KLH (100 μg) adsorbed to alum. Splenic cryosections were obtained 9 d after challenge and stained with PNA ( purple) and anti-B220 antibodies (pink). Stained cryosections from a representative p52/p100 (−/−) marrow transferred animal is shown.