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. 1998 Jan 19;187(2):245–251. doi: 10.1084/jem.187.2.245

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The absence of Ii chain and H-2M results in synergistic reduction of CD4⁺ T cell development. Thymocytes from (A) wild-type B6x129 and mutant (B) Ii^−/−, (C) Ii^−/−M^−/−, (D) M^−/−, and (E) MHC-II^−/− mice were analyzed for expression of CD4, CD8, and TCR-α/β using flow cytometry. Shown are two parameter dot plots of CD8 versus TCR-α/β, in which three electronic gates are indicated, delineating the following populations: CD4⁻CD8^hiTCR^hi (box 1), CD4⁺CD8⁻TCR^hi (box 2), and CD4⁺CD8^loTCR^lo (box 3). Also shown are the percentages of cells that fall within each box. Cells in boxes 2 and 3 are >97% CD4⁺. Splenocytes from wild-type and mutant mice were analyzed for expression of CD4, CD8, and TCR-α/β using flow cytometry. Shown are comparisons of TCR expression on (F) CD4⁺ T cells and (G) CD8⁺ T cells in the mice of the five genotypes, as indicated. Data are representative of analyses of four mice of each genotype.

The absence of Ii chain and H-2M results in synergistic reduction of CD4⁺ T cell development. Thymocytes from (A) wild-type B6x129 and mutant (B) Ii^−/−, (C) Ii^−/−M^−/−, (D) M^−/−, and (E) MHC-II^−/− mice were analyzed for expression of CD4, CD8, and TCR-α/β using flow cytometry. Shown are two parameter dot plots of CD8 versus TCR-α/β, in which three electronic gates are indicated, delineating the following populations: CD4⁻CD8^hiTCR^hi (box 1), CD4⁺CD8⁻TCR^hi (box 2), and CD4⁺CD8^loTCR^lo (box 3). Also shown are the percentages of cells that fall within each box. Cells in boxes 2 and 3 are >97% CD4⁺. Splenocytes from wild-type and mutant mice were analyzed for expression of CD4, CD8, and TCR-α/β using flow cytometry. Shown are comparisons of TCR expression on (F) CD4⁺ T cells and (G) CD8⁺ T cells in the mice of the five genotypes, as indicated. Data are representative of analyses of four mice of each genotype.