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. 1998 Jan 19;187(2):185–196. doi: 10.1084/jem.187.2.185

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In vitro proliferation of B cells lacking NF-κB2. Purified resting B cells from nfkb2-null (open circles) and control mice (closed circles) were cultured during 72 h at 10⁵ cells/ml in the presence of different concentrations of (A) LPS (0.09–20 μg/ml); (B) anti–IgD-dextran (0.1–10 ng/ml); or (C) anti-CD40 mAb (0.08–5 μg/ml) + IL-4 (3,000 U/ml) + IL-5 (150 U/ml). [³H]thymidine was added for 12 h and cells were then harvested for determination of [³H]thymidine incorporation. Values shown represent the means of triplicate cultures ± SEM.