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. 1998 Jan 19;187(2):237–244. doi: 10.1084/jem.187.2.237

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ERK activation by cross-linking of full-length, but not that by CD40Δ246, was prominently suppressed by dominant negative mutant of Ras. Cells (10⁶) were cotransfected with ERK– (A) or NFκB–dependent reporter (B) and either 1 μg of full-length CD40 (a) or CD40Δ246 (b), together with 5 μg of N17Ras, DNRaf, and MEK^DN. Total amount of DNA was adjusted with empty vector at 10 μg. Cells were stimulated with anti-CD40 mAb 24 h after transfection. After 24 h incubation, cell lysates were subjected to luciferase assay. Relative activation of ERK and NFκB in each transfection was calculated as described above. The mean value of two to three independent experiments (column) was calculated from a mean value of a single experiment in triplicate (closed circle).