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. 1998 Jan 19;187(2):265–270. doi: 10.1084/jem.187.2.265

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TNF-α–release assays after stimulation of the CTL line NW38-IVS-1 by COS-7 cell transfectants. TNF release was detected against COS-7 cells cotransfected with the expression vector pcDNA3.1 (−) containing NY-ESO-1 cDNA, and pcDNA1Amp containing HLA-A2 cDNA. No TNF release was detected against COS-7 transfected with vector alone, vector/HLA-A1, vector/HLA-A2, vector/NY-ESO-1, and vector/NY-ESO-1/HLA-A1.