Figure 3.

Production of IFN-γ and IL-2 in IL-4^−/− (solid columns) and IL-4^+/+ (dotted columns) mice infected with 10⁶ PCA-2 cells intravenously (Systemic infection) or 10⁸ CA-6 intragastrically (Gastrointestinal infection). Purified CD4⁺ T splenocytes were obtained at 4 d after reinfection with 5 × 10⁵ CA-6 cells, 14 d after primary infection. Cells were cultured in the presence of Candida antigen and macrophages as accessory cells, as described in Materials and Methods. Cytokine levels in unstimulated cultures were below the detection limit of the assay, as indicated on the y-axis.