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. 1998 Feb 2;187(3):307–317. doi: 10.1084/jem.187.3.307

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Expression of IL-12 and IL-12R genes in IL-4^−/− and IL-4^+/+ mice infected with C. albicans. Purified splenic macrophages, peritoneal neutrophils, and CD4⁺ splenocytes were obtained from uninfected mice (lane 1) or from PCA-2–infected mice 3 d after reinfection with 10⁶ CA-6 cells (lane 2). PCA-2–infected mice were treated with rIL-4 (IL-4^−/− mice) or with anti-IL–4 mAb (IL-4^+/+) before reinfection (lane 3), as described in Table 3. Cytokine gene expression was assessed by RT-PCR. N, no DNA added to the amplification mix during PCR; C, HPRT- or cytokine-specific controls.