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. 1998 Feb 2;187(3):307–317. doi: 10.1084/jem.187.3.307

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Effect of IL-4 on cytokine production (A) and IL-4R expression (B) on neutrophils and macrophages from IL-4^−/− (solid columns) or IL-4^+/+ (dotted columns) mice. Elicited peritoneal neutrophils or macrophages were cultured in vitro with IFN-γ + LPS or C. albicans cells. Priming with IL-4 was done by adding 250 U/ml of rIL-4 8 h before C. albicans (striped columns, A; and lane 2, B). After 24 h incubation, supernatants were assayed for cytokine contents and cells for IL-4R expression by RT-PCR. *, Cytokine levels below the detection limit of the assay, as indicated on the y-axis. Lane 1, C. albicans alone. N, No DNA added to the amplification mix during PCR; C, HPRT- or cytokine-specific controls.

Effect of IL-4 on cytokine production (A) and IL-4R expression (B) on neutrophils and macrophages from IL-4^−/− (solid columns) or IL-4^+/+ (dotted columns) mice. Elicited peritoneal neutrophils or macrophages were cultured in vitro with IFN-γ + LPS or C. albicans cells. Priming with IL-4 was done by adding 250 U/ml of rIL-4 8 h before C. albicans (striped columns, A; and lane 2, B). After 24 h incubation, supernatants were assayed for cytokine contents and cells for IL-4R expression by RT-PCR. *, Cytokine levels below the detection limit of the assay, as indicated on the y-axis. Lane 1, C. albicans alone. N, No DNA added to the amplification mix during PCR; C, HPRT- or cytokine-specific controls.