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. 1998 Feb 2;187(3):329–339. doi: 10.1084/jem.187.3.329

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Platelet adhesion to HUVEC monolayers after addition of adhesive proteins. Thrombin-activated platelets were allowed to adhere to HUVEC monolayers for 30 min in the presence of different adhesive proteins. Platelet binding was then determined by flow cytometry as described above. The following concentrations were used: 4 mg/ml albumin, 75 μg/ ml fibrinogen, 15 μg/ml fibronectin, and 0.25 μg/ml vWF, which corresponded to a plasma concentration of 10, 2.5, 5, and 2.5%, respectively. Ristocetin was added together with vWF at a final concentration of 1 mg/ml. Results are expressed as the mean ± SD of the median fluorescence (FL-1-H) of at least four experiments. **P <0.05 by Student's t test, compared with adhesion without proteins.