Table 4.

Comparison of Hydrogen-bonded Antibody–Antigen Contact Distances in HuLys and D1.3

Fv atom		Lysozme atom		D1.3 distance		HuLys distance (molecules 1,2)
				Å		Å
Ly30 His ND1		129 Leu OT2		3.4		3.3, 4.6
L50 Tyr OH		18 Asp OD1		3.2		3.2, 3.7
L50 Tyr OH		18 Asp OD2		2.7		2.7, 2.9
L53 Thr OG1		19 Asn ND2		2.8		3.2, 3.1
L91 Phe O		121 Gln NE2		2.8		3.0, 3.0
L93 Ser N		121 Gln OE1		2.9		2.9, 2.8
H53 Gly N		117 Gly O		2.8		3.1, 3.3
H96 Arg NH2		102 Gly O		2.7		2.8, 4.0
H97 Asp OD1		24 Ser OG		2.8		2.8, 3.2
H97 Asp OD1		27 Asn ND2		3.1		3.5, 3.1
H97 Asp OD2		22 Gly O		3.0		3.0, 3.0
H97 Asp OD2		24 Ser N		2.9		2.9, 3.0
H98 Tyr OH		119 Asp OD1		2.7		2.9, 2.7
H98 Tyr OH		120 Val N		3.2		3.3, 3.2
H98 Tyr OH		121 Gln N		3.0		3.2, 2.9
H99 Arg NH1		22 Gly O		2.8		3.0, 3.0
H99 Arg NH2		22 Gly O		3.4		2.7, 3.2

All direct D1.3-lysozyme hydrogen bonds shorter than 3.5 Å are listed. These residues are a subset of a larger list, which includes residues that hydrogen bond via water molecules.